Peer-reviewed articles

2022

62. Niklas Sandström*, Valentina Carannante*, Karl Olofsson, Patrick A. Sandoz, Elisabeth Moussaud-Lamodière, Brinton Seashore-Ludlow, Hanna Van Ooijen, Quentin Verron, Thomas Frisk, Madoka Takai, Martin Wiklund, Päivi Östling and Björn Önfelt (2022) Miniaturized and multiplexed high-content screening of drug and immune sensitivity in a novel multichambered microwell chip, Cell Reports Methods 2(7), 100256

61. Apple Tay Hui Min, Rubén Prieto-Díaz, Shiyong Neo, Le Tong, Xinsong Chen, Valentina Carannante, Björn Önfelt, Johan Hartman, Felix Haglund, Maria Majellaro, Jhonny Azuaje, Xerardo Garcia-Mera, Jose M. Brea, Maria I. Loza, Willem Jespers, Hugo Gutierrez-de-Teran, Eddy Sotelo and Andreas Lundqvist (2022) A2B Adenosine Receptor Antagonists Rescue Lymphocyte Activity in Adenosine-Producing Patient-Derived Cancer Models. The Journal for ImmunoTherapy of Cancer 10(5). E004592

60. Lucas C.M. Arruda, Arwen Stikvoort, Melanie Lambert, Liqing Jin, Laura Sanchez Rivera, Renato M.P. Alves, Tales Rocha De Moura, Carsten Mim, Sören Lehmann, Rebecca Axelsson-Robertson, John E. Dick, Jonas Mattsson, Björn Önfelt, Mattias Carlsten, Michael Uhlin (2022) A novel CD34-specific T-cell engager efficiently depletes acute myeloid leukemia and leukemic stem cells in vitro and in vivo. Haematologica 107(8):1786-1795

59. Niklas Sandström, Ludwig Brandt, Patrick Alain Sandoz, Chiara Zambarda, Karolin Guldevall, Malte Schulz-Ruhtenberg, Bernd Rösener, Robin Alexander Krüger, Björn Önfelt (2022) Live single cell imaging assays in glass microwells produced by laser-induced deep etching. Lab on a chip, 22, 2107-2121

58. Karoliina Tuomela, Debayan Mukherjee, Ashley R Ambrose, Ashish Harikrishnan, Holly Mole, Adam Hurlstone, Björn Önfelt, Jamie Honeychurch, Daniel M Davis (2022) Radiotherapy transiently reduces the sensitivity of cancer cells to lymphocyte cytotoxicity, PNAS, 119:3

2021

57. Karl Olofsson, Valentina Carannante, Madoka Takai, Björn Önfelt and Martin Wiklund (2021) Single cell organization and cell cycle characterization of DNA stained multicellular tumor spheroids, Scientific Reports 11, 17076

56. Quentin Verron, Elin Forslund, Ludwig Brandt, Mattias Leino, Thomas W. Frisk, Per E. Olofsson and Björn Önfelt (2021) NK cells integrate signals when building immune synapses but require local stimuli for degranulation, Science Signaling 14(684):eabe2740 LINK TO FULL TEXT VERSION

55. Karl Olofsson, Valentina Carannante, Madoka Takai, Björn Önfelt and Martin Wiklund (2021) Ultrasound-based scaffold-free core-shell multicellular tumor spheroid formation. Micromachines 12(3):329

54. Alexandros Karampatzakis, Petr Broz, Ashley Ambrose, Björn Önfelt, Gabriela Dos Santoz Cruz De Matos, Daniel Rycroft and Daniel M. Davis (2021) Antibody afucosylation augments CD16-mediated serial killing and IFNγ secretion by human Natural Killer cells. Frontiers in Immunology 12:641521

53. Jolien Vandermeer, Maas RJA, Guldevall K, Klarenaar K, Geluk HCA, Cany J, De Jonge PKJD, Wagena E, Friedl P, Önfelt B, Massuger LF, Jansen JH, Hobo W, Dolstra H (2021) IL-15 superagonist N-803 improves IFNγ production and killing of leukemia and ovarian cancer cells by CD34+ progenitor-derived NK cells. Cancer Immunology Immunotherapy 70(5):1305-1321

2020

52. S.J. Edwards, V. Carannante, K. Kuhnigk, H. Ring, T. Tararuk, F. Hallböök, H. Blom, B. Önfelt, H. Brismar (2020) High-resolution imaging of tumour spheroids and organoids enabled by expansion microscopy. Frontiers in Molecular Biosciences 7:208

51. J. Dunst, V. Glaros, L. Englmaier, P. Sandoz, B. Önfelt, J. Kisielow, and T. Kreslavsky (2020) Recognition of synthetic polyanionic ligands underlies 'spontaneous' reactivity of Vγ1 γδTCRs. Journal of Leukocyte Biology 107(6):1033-1044

2019

50. C. L. Fleming, P. A. Sandoz, T. Inghardt, B. Önfelt, M. Grøtli and J. Andreasson (2019) A Fluorescent LCK Inhibitor that Exhibits Diagnostic Changes in Emission Upon Binding the Kinase Enzyme. Angewandte Chemie International Edition, 58(42):15000-15004

49. I. Prager, C. Liesche, H. van Ooijen, D. Urlaub, Q. Verron, N. Sandström, F. Fasbender, M. Claus, R. Eils, J. Beaudouin, B. Önfelt,C. Watzl (2019) Natural Killer cells switch from Granzyme B to death receptor-mediated cytotoxicity during serial killing of tumor cells. Journal of Experimental Medicine 216(9):2113-2127

48. P. E. Olofsson, L. Brandt, K.L.E. Magnusson, T. Frisk, J. Jaldén and B. Önfelt. (2019) A collagen-based microwell assay to study NK-target cell interactions. Scientific Reports, 9, 10672

47. A. Gaballa, A. Stikvoort, B. Önfelt, J. Mattsson, M. Sundin, E. Watz, and M. Uhlin (2019) Frequencies of CD8+γδ and CD27+γδ cells in the stem cell graft predict the outcome after allogeneic hematopoietic cell transplantation, Bone Marrow Transplantation, Published online feb 5, 201

46. E. Rådestad, M. Sundin, J. Törlén, S. Thunberg, B. Önfelt, P. Ljungman, E. Watz, J. Mattsson and M. Uhlin. (2019) Individualization of Hematopoietic Stem Cell Transplantation Using Alpha/Beta T-Cell Depletion, Frontiers in Immunology, 10:189

2018

45. K. Walwyn-Brown, K. Guldevall, M. Saeed, D. Pende, B. Önfelt, A.S. MacDonald, D.M. Davis (2018) Human NK cells lyse Th2-polarizing dendritic cells via NKp30 and DNAM-1. Journal of Immunology 201(7):2028-2041

44. K. Olofsson, V. Carannante, M. Ohlin, T Frisk, K. Kushiro, M. Takai, A. Lundqvist, B. Önfelt and M. Wiklund. (2018) Acoustic formation of multicellular tumor spheroids enabling on-chip functional structural imaging. Lab on a Chip 18(16):2466-2476

43. K. Srpan, A. Ambrose, A. Karampatzakis, M. Saeed, A.N.R. Cartwright, K. Guldevall, G. D. S. Cruz De Matos, B. Önfelt and D.M. Davis. (2018) Shedding of CD16 Disassembles the NK Cell Immune Synapse and Boosts Serial Engagement of Target Cells. J. Cell Biol. 217(9):3267-3283

42. D. Sarhan, L. Brandt, M. Felices, K. Guldevall, T. Lenvik, J.M. Curtsinger, E.D. Warlick, S.R. Spellman, B. R Blazar, D.J. Weisdorf, S. Cooley, D.A. Vallera, B. Önfelt and J. Miller. (2018) 161533 TriKE restores NK cell dysfunction mediated by Myeloid-derived suppressor cells in Myelodyspastic patients Blood Advances 2(12):1459-1469

41. V.Y.S. Oei, M. Siernicka, A. Gracyk-Jarzynka, H.J. Hoel, W. Yang, D. Palacios, H. Almåsbak, M. Bajor, L. Brandt, B. Önfelt, J. Goodridge, M. Winiarska, R. Zagozdzon, J. Olweus, J.A. Kyte, K.J. Malmberg. (2018) Intrinsic functional potential of NK-cell subsets constrains retargeting driven by chimeric antigen receptors. Cancer Immunol Res 6(4):467-480

40. A. Stikvoort, A. Gaballa, M. Solders, I Nederlof, B. Önfelt, B. Sundberg, M. Remberger, M. Sundin, J. Mattssonand M. Uhlin. (2018) Risk factors for severe acute graft-versus-host disease in donor graft composition. Biol blood Marrow Transplant. 24(3):467-477

2017

39. E. Fogelqvist, M. Kördel, V. Caranannte, B. Önfelt and H. Hertz. (2017) Laboratory cryo x-ray microscopy for 3D cell imaging. Sci Rep 7(1):13433

2016

38. H-T. Hsu, D. Viswanath, E. Mace, A. Christakou, M. Wiklund, B. Önfelt and J. Orange. (2016) Lytic granule convergence is essential for NK cells to promote targeted killing while preventing collateral damage. The Journal of Cell Biology 215(6):875-889

37. K. Guldevall, L. Brandt, E. Forslund, K. Olofsson, T. Frisk, P. E. Olofsson, K. Gustafsson, B. Vanherberghen, O. Manneberg, H. Brismar, K. Kärre, M. Uhlin and B. Önfelt. (2016) Microchip screening platform for single cell assessment of NK cell cytotoxicity, Front. Immunol 7:119.

2015

36. E. Forslund, E. Sohlberg, M. Enqvist, P.E. Olofsson, K.J. Malmberg and B. Önfelt (2015) Microchip-based single-cell imaging reveals that CD56dimCD57-KIR-NKG2A+ NK cells have more dynamic migration associated with increased target cell conjugation and probability of killing compared to CD56dimCD57-KIR-NKG2A- NK cells. J. Immunol. 195(7):3374-3381

35. A.E. Christakou, M. Ohlin, B. Önfelt, M. Wiklund (2015) Ultrasonic three-dimensional on-chip cell culture for dynamic studies of tumor immune surveillance by natural killer cells. Lab. Chip. 15(15): 3222-3231

34. J. Tauriainen, K. Gustafsson, M. Göthlin, J. Gertow, M. Buggert, T. Frisk, A.C. Karlsson, M. Uhlin and B. Önfelt (2015) Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7. Front. Immunol 6:196

33. Monika Enqvist, Eivind Heggernes Ask, Elin Forslund, Mattias Carlsten, Greger Abrahamsen, Vivien Béziat, Sandra Andersson, Marie Schaffer, Anne Spurkland, Yenan Bryceson, Björn Önfelt and Karl-Johan Malmberg (2015) Co-ordinated Expression of DNAM-1 and LFA-1 in Educated NK Cells. J. Immunol. 194(9): 4518-4527

2014

32. P.E. Olofsson, E. Forslund, B. Vanherberghen, K. Chechet, O. Mickelin, A. Rivera Ahlin, T. Everhorn and B. Önfelt (2014) Distinct migration and contact dynamics of resting and IL-2-activated human natural killer cells. Front. Immunol. 5:80.

2013

31. M. Sternberg-Simon, P. Brodin, Y. Pickman, B. Önfelt, K Kärre, KJ Malmberg, P Höglund and R. Mehr (2013) Natural killer cell inhibitory receptor expression in human and mice: a closer look, Front. Immunol. 4:65.

30. A. Christakou, M. Ohlin, B. Vanherberghen, M. A. Khorshidi, M. Kadri, T. Frisk, M. Wiklund and B. Önfelt (2013) Live Cell Imaging in a Micro-Array of Acoustic Traps Facilitate Quantification of Natural Killer Cell Heterogeneity, Integr. Biol., 5(4), 712 – 719

29. B Vanherberghen, P. E. Olofsson, E. Forslund, M. Sternberg-Simon, M. A. Khorshidi, S. Pacouret, K. Guldevall, M. Enqvist, K-J. Malmberg, R. Mehr, B. Önfelt. (2013) Classification of human natural killer cells based on migration behavior and cytotoxic response. Blood, 121(8), 1326-1334

28. M. Ohlin, A. E. Christakou, T. Frisk, B. Önfelt, M. Wiklund (2013) Influence of acoustic streaming on ultrasonic particle manipulation in a 100-well ring-transducer microplate. J. Micromec. Microeng. 23(3):035008

2012

27. K. Wilkinson, B. Ekstrand-Hammarström, L. Ahlinder, K. Guldevall, R. Pazik, L. Kępiński, K. O. Kvashnina, S. M. Butorin, H. Brismar, B. Önfelt, L. Österlund, G. A. Seisenbaeva and V. G. Kessler. (2012) Visualization of custom-tailored iron oxide nanoparticles chemistry, uptake, and toxicity. Nanoscale. 4(23):7383-7393

26. E. Forslund, K. Guldevall, P.E. Olofsson, T. Frisk, A.E. Christakou, M. Wiklund and B. Önfelt. (2012) Novel Microchip-Based Tools Facilitating Live Cell Imaging and Assessment of Functional Heterogeneity within NK Cell Populations. Front Immunol. 3:300.

25. L. Perisic, M. Lal, J. Hulkko, K. Hultenby, B. Önfelt, Y. Sun, F. Dunér, J. Patrakka, C. Betsholtz, M. Uhlen, H. Brismar, K. Tryggvason, A. Wernerson and T. Pikkarainen (2012) Plekhh2, a novel podocyte protein downregulated in human focal segmental glomerulosclerosis, is involved in matrix adhesion and actin dynamics. Kidney Int. 82(10):1071-1083

2011

24. J.R. Nilsson, S. Li, B. Önfelt, J. Andréasson (2011) Light-Induced Cytotoxicity of a Photochromic Spiropyran. Chemical Communications 47 (39),11020-11022

23. M.A. Khorshidi, B. Vanherberghen, J.M. Kowalewski, K.R. Garrod, S. Lindström, H. Andersson-Svahn, H. Brismar, M.D. Cahalan, B. Önfelt (2011) Analysis of Transient Migration Behavior of Natural Killer Cells Imaged in situ and in vitro. Integrative Biology 3: 770-778

22. T. Frisk, M.A. Khorshidi, K Guldevall, B. Vanherberghen, B. Önfelt (2011) A Silicon-Glass Microwell Platform for High-Resolution Imaging and High-Content Screening with Single Cell Resolution. Biomedical microdevices 13: 683-693

2010

21. K. Guldevall, B. Vanherberghen, T. Frisk, J. Hurtig, A. Christakou, O. Manneberg, S. Lindström, H. Andersson-Svahn, M. Wiklund, B. Önfelt (2010) Imaging immune surveillance of individual natural killer cells confined in microwell arrays. PLoS ONE 5: e15453.

20. B. Vanherberghen, O. Manneberg, A. Christakou, T. Frisk, M. Ohlin, H.M. Hertz, B. Önfelt, M. Wiklund (2010) Ultrasound-controlled cell aggregation in a multi-well chip. Lab on a Chip 10: 2727-32.

2009

19. R.K.P. Benninger, B. Vanherberghen, S. Young, S.B. Taner, F.J. Culley, T. Schnyder, M.A.A. Neil, D. Wüstner, P.M.W. French, D.M. Davis, B. Önfelt (2009) Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of Natural Killer cell immune synapses Biophysical Journal 96: L13-15.

18. O. Manneberg, B. Vanherberghen, B. Önfelt, M. Wiklund (2009) Flow-free transport of cells in microchannels by frequency-modulated ultrasound. Lab on a Chip 9: 833 - 837.

2008

17. O. Manneberg, B. Vanherberghen, J. Svennebring, H.M. Hertz, B. Önfelt, M Wiklund. (2008) A three-dimensional ultrasonic cage for characterization of individual cells. Applied Physics Letters 93: 063901.

From Postdoc period

16. S. Sowinski, C. Jolly, O. Berninghausen, M.A. Purbhoo, A. Chauveau, K. Kohler, S. Oddos, P. Eissmann, F.M. Brodsky, C. Hopkins, B. Önfelt, Q. Sattentau D.M. Davis (2008) Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission. Nature Cell Biology. 10: 211-219.

15. R.K.P. Benninger, O. Hofmann, B. Önfelt, D.M. Davis, M.A.A. Neil, P.M.W. French, A.J. deMello (2007) Fluorescence lifetime imaging of Dye: DNA interactions within continuous flow microfluidic systems. Angewante Chemie International Edition 119: 2278-2281.

14. F.E. McCann, P. Eissmann, B. Önfelt, R. Leung, D.M. Davis (2007) The activating NKG2D ligand MICA transfers from target cells to NK cells in a manner that allows functional consequences. Journal of Immunology 178: 3418-3426.

13. B. Önfelt, S. Nedvetzki, R.K.P. Benninger, M.A. Purbhoo, S. Sowinski, A.N. Hume, M.C. Seabra, M.A.A. Neil, P.M.W. French, D.M. Davis (2006) Structurally distinct membrane nanotubes between human macrophages support long-distance vesicular traffic or surfing of bacteria. Journal of Immunology 177: 8476-8483.

12. R.K.P. Benninger*, B. Önfelt*, M.A.A. Neil, D.M. Davis, P.M.W. French (2005) Fluorescence imaging of two-photon linear dichroism: cholesterol depletion disrupts molecular orientation in cell membranes. Biophysical Journal 88: 609-622.

11. C. Dunsby, P.M.P. Lanigan, J. McGinty, D.S. Elson, J. Requejo-Isidro, I. Munro, N. Galletly, F.E. McCann, B. Treanor, B. Önfelt, D.M. Davis, M.A.A. Neil, P.M.W. French (2004) An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy. Journal of Physics D-Applied Physics 37: 3296-3303.

10. B. Önfelt, S. Nedvetzki, K. Yanagi, D.M. Davis Cutting edge: (2004) Membrane nanotubes connect immune cells. Journal of Immunology 173: 1511-1513.

9. K. Eleme*, S.B. Taner*, B. Önfelt*, L.M. Collinson, F.E. McCann, N.J. Chalupny, D. Cosman, C. Hopkins, A.I. Magee, D.M. Davis (2004) Cell surface organization of stress-inducible proteins ULBP and MICA that stimulate human NK cells and T cells via NKG2D. Journal of Experimental Medicine 199: 1005-1010.

From Ph.D. period

8. J. Olofsson, B. Önfelt, P. Lincoln (2004) Three-state light switch of Ru(phen)2dppz2+: Distinct excited-state species with two, one, or no hydrogen bonds from solvent. Journal of Physical Chemistry A 108: 4391-4398.

7. B. Önfelt, J. Olofsson, P. Lincoln, B. Norden (2003) Picosecond and steady-state emission of Ru(phen)2dppz2+ in glycerol: Anomalous temperature dependence. Journal of Physical Chemistry A 107: 1000-1009.

6. B. Önfelt, L. Göstring, P. Lincoln, B. Norden, A. Önfelt (2002) Cell studies of the DNA bis-intercalator Delta-Delta µ- C4(cpdppz)2-(phen)4Ru-24+: toxic effects and properties as a light emitting DNA probe in V79 Chinese hamster cells. Mutagenesis 17: 317-320.

5. J. Olofsson, B. Önfelt, P. Lincoln, B. Norden, P. Matousek, A.W. Parker, E. Tuite (2002) Picosecond Kerr-gated time-resolved resonance Raman spectroscopy of the Ru(phen)2dppz2+ interaction with DNA. Journal of Inorganic Biochemistry 91: 286-297.

4. B. Önfelt, P. Lincoln, B. Norden (2001) Enantioselective DNA threading dynamics by phenazine-linked Ru(phen)2dppz2+ dimers. Journal of the American Chemical Society 123: 3630-3637.

3. C.G. Coates, J. Olofsson, M. Coletti, J.J. McGarvey, B. Önfelt, P. Lincoln, B. Norden, E. Tuite, P. Matousek, A.W. Parker (2001) Picosecond time-resolved resonance Raman probing of the light- switch states of Ru(phen)2dppz2+ Journal of Physical Chemistry B 105: 12653-12664.

2. B. Önfelt, P. Lincoln, B. Norden, J.S. Baskin A.H. Zewail (2001) Femtosecond linear dichroism of DNA-intercalating chromophores: Solvation and charge separation dynamics of [Ru(phen)(2)dppz](2+) systems. Proceedings of the National Academy of the USA 97: 5708-5713.

1. B. Önfelt, P. Lincoln, B. Norden (1999) A molecular staple for DNA: Threading bis-intercalating [Ru(phen)(2)dppz](2+) dimer. Journal of the American Chemical Society 121: 10846-10847.

Review articles 

9. J.P. Goodridge, B. Önfelt, K.J. Malmberg (2015) Newtonian cell interactions shape natural killer cell education. Immunological Reviews 267(1):197-213

8. M. Wiklund, A.E. Christakou, M. Ohlin, I. Iranmanesh, T. Frisk, B. Vanherberghen, B. Önfelt (2014) Ultrasound-Induced Cell–Cell Interaction Studies in a Multi-Well Microplate Micromachines. 5:27-49.

7. I Parmryd I and B. Önfelt (2013) Consequences of membrane topography. FEBS J. 280(12): 2775-2784

6. M. Wiklund, H. Brismar and B. Önfelt (2012) Acoustofluidics 18: Microscopy for acoustofluidic micro-devices. Lab Chip. 12(18):3221-3234.

5. M. Wiklund, B. Önfelt (2012) Ultrasonic manipulation of single cells. In: Single-cell Analyses, Methods in Molecular Biology, Humana Press, Springer Group, USA. 853: 177-196

4. J. Hurtig, D.T. Chiu, B. Önfelt (2010) Intercellular nanotubes: insights from imaging studies and beyond. Wiley Interdisciplinary Reviews Nanomedicine Nanobiotechnology, 2: 260-276.

3. B. Önfelt, M.A. Purbhoo, S. Nedvetzki, S. Sowinski, D.M. Davis (2005) Long-distance calls between cells connected by tunneling nanotubules. Science STKE 2005: pe55

2. B. Önfelt, D.M. Davis (2004) Can membrane nanotubes facilitate communication between immune cells? Biochemical Society Transactions 32: 676-678

1. S.B. Taner, B. Önfelt, N.J. Pirinen, F.E. McCann, A.I. Magee, D.M. Davis (2004) Control of immune responses by trafficking cell surface proteins, vesicles and lipid rafts to and from the immunological synapse. Traffic 5: 651-661

Book chapters

4. B. Vanherberghen, T. Frisk, E. Forslund, P.E. Olofsson, K. Guldevall, and B. Önfelt (2016) Live cell imaging of single cells to assess NK cell heterogeneity, Methods Mol Biol. 1441:87-106

3. M. Wiklund, H. Brismar and B. Önfelt (2015) Microscopy for acoustofluidics micro-devices. Microscale Acoustofluidics, Royal Society of Chemistry, London, Chapter 19 493-519

2. M. Wiklund, B. Önfelt (2012) Ultrasonic manipulation of single cells. In: Single-cell Analyses, Methods in Molecular Biology, Humana Press, Springer Group, USA. 853: 177-196

1. T. Lobovkina, A. Jesorka, B. Önfelt, J. Lagerwall, O. Orwar (2009) Soft-Matter Nanotubes, In: Nanotubes, Springer Verlag Berlin - Heidelberg